Fig 1: Effect of hypoxia and Wnt on the expression of cementogenic marker, CEMP1, in PDLCs in normoxic and hypoxic (1 mM dimethyloxalylglycine) conditions, with or without Wnt-CM. (A) Western blot analysis of CEMP1 protein expression levels in DMEM, with quantification relative to a-Tubulin. (B) RT-qPCR analysis of CEMP1 mRNA expression levels in DMEM, with quantification relative to GAPDH. (C) Western blot analysis of CEMP1 protein expression levels in OM, with quantification relative to a-Tubulin. (D) RT-qPCR of CEMP1 mRNA expression levels in OM, with quantification relative to GAPDH. PDLC, periodontal ligament cells; OM, osteogenic medium; DMEM, Dulbecco's modified Eagle's medium; CM, conditioned medium; CEMP1, cementum protein 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Supplier Page from Abcam for Anti-CEMP1 antibody